RAPID ISOCRATIC REVERSED-PHASE HPLC OF TAXANES ON NEW COLUMNS DEVELOPED SPECIFICALLY FOR TAXOL ANALYSIS

We describe a new HPLC method for detection of taxanes from crude extr acts, using new specialty columns developed for HPLC analysis. Taxol [ 1] and cephalomannine [2] were separated in 13 minutes using an isocra tic elution. Additionally, there is baseline separation between cephal omannine [2], 7-epi-10-deacetyl-taxol [3], and taxol [1]. Elution of 7 -epi-taxol [4] occurs in 20 minutes. We compared chromatography of tax anes on specialty, C18, and phenyl columns. This isocratic HPLC method permits rapid analysis of large numbers of crude extracts, with excel lent resolution of taxanes, enabling the detection of 7-epitaxol [4] i n 20 minutes.