SURVEY OF METHODS FOR MEASURING HUMAN ANTI-MOUSE ANTIBODIES

Human anti-mouse antibodies (HAMA) methods were surveyed using a panel of 17 serum and two plasma specimens which were circulated to ten lab oratories for analysis. The panel included HAMA-negative and HAMA-posi tive specimens (from patients given a variety of mouse monoclonal anti bodies) and a specimen with a high rheumatoid factor titer. The partic ipating laboratories used a diverse range of methods to determine HAMA and all three of the commercially available quantitative methods for HAMA were represented. The survey revealed significant differences in HAMA results among the different laboratories.