APPRAISAL OF FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) TECHNIQUES IN PRENATAL-DIAGNOSIS

Authors
GRAO P GUITART M COLL MD EGOZCUE J
Citation
P. Grao et al., APPRAISAL OF FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) TECHNIQUES IN PRENATAL-DIAGNOSIS, Early human development, 33(2), 1993, pp. 101-108
Citations number
20
Categorie Soggetti
Obsetric & Gynecology",Pediatrics
Journal title
Early human development → ACNP
ISSN journal
03783782
Volume
33
Issue
2
Year of publication
1993
Pages
101 - 108
Database
ISI
SICI code
0378-3782(1993)33:2<101:AOFIH(>2.0.ZU;2-F
Abstract
The reliability of FISH was appraised using probes for the X and Y chr omosome and for chromosomes 12 and 18 in prenatal and adult interphase nuclei. Detection of a single hybridization spot proved to be quite r eliable (80-92% positive nuclei). Detection of two hybridization spots was more difficult; percentages of nuclei showing two signals varied between 62-72%. The percentages of nuclei with the correct number of s pots was higher in the metaphases occasionally found. Thus, FISH may c omplement but not replace cytogenetic analysis. For sex determination and for the detection of mosaicism, we suggest the use of two differen t probes in separate regions of the same preparation.