LOCALIZATION OF THE B-HORDEIN LOCUS ON BARLEY CHROMOSOMES USING FLUORESCENCE IN-SITU HYBRIDIZATION

The B-hordein gene family consists of at least 13 genes that appear to be clustered in two regions on the barley chromosome 1H (5). Using fl uorescence in situ hybridization techniques, we have localized the B-h ordein locus (Hor2) to the distal end of the short arm of chromosome 1 H (5), corresponding to the genetically determined position, 91% dista l on the short arm. This result is based on appropriate barley lines ( i.e. the two cultivars Igri and Betzes, the telotrisomic and ditelotet rasomic lines 1HS and the translocation line T21), on two experimental approaches and on a signal frequency of between 20% and 60% on metaph ase chromosomes.