CO2 production was followed by manometry in starved cell-free extracts
of fission yeast stimulated by unstarved cell-free extracts from a sy
nchronous culture. The degree of stimulus, measured by the lag time in
CO2 production, varied markedly during the cell cycle, with a maximum
for cells at about mitosis and a minimum for septated cells. Similar
differences in lag time were found with unstarved extracts of cdc13.11
7 grown at 37-degrees-C and 35-degrees-C.