SPIN-TRAPPING AND CHEMILUMINESCENCE STUDIES OF NEUTROPHILS FROM A HOLSTEIN-FRIESIAN CALF WITH BOVINE LEUKOCYTE ADHESION DEFICIENCY

The ability of neutrophils from a Holstein-Friesian calf with bovine l eukocyte adhesion deficiency (the proband with a genetic deficiency of the Mac-1 (CD11b/CD18) glycoprotein corresponding to the receptor of complement iC3b) to generate oxygen radicals was examined using electr on spin resonance spectrometry (ESR) combined with a spin-trapping tec hnique and luminol-dependent chemiluminescence spectrometry. When the neutrophils were stimulated with phorbol 12-myristate 13-acetate (PMA) , an ESR spectrum confirming the generation of superoxide anions (O2-) was clearly observed in both healthy and diseased calves. However, wh en the neutrophils were stimulated by opsonized zymosan, appearance of the ESR spectrum was recognized in the healthy calves but not in the diseased calf. Similar results were obtained from chemiluminescence ex periments.