Jm. Tosk et al., MODULATION OF CHEMILUMINESCENCE IN A MURINE MACROPHAGE CELL-LINE BY NEUROENDOCRINE HORMONES, International journal of immunopharmacology, 15(5), 1993, pp. 615-620
This study determined the effects of neuropeptides and neuroendocrine
hormones at the cellular level of the immune response using a murine m
acrophage cell line, J774, which exhibits a chemiluminescent oxidative
burst upon acute stimulation with zymosan. We report that the zymosan
-triggered oxidative burst of J774 cells can be modulated by the opioi
d peptides beta-endorphin (beta-END) and dynorphin A (DYN) in a naloxo
ne-reversible fashion. Norepinephrine (NE) also modulated chemilumines
cence (CL) emission of J774 cells, with dose-dependent suppression of
CL dependent upon co-incubation with gamma-interferon (gamma-INF). Wit
hout gamma-INF co-incubation, NE shared with the opioid peptides beta-
END and DYN the ability to modulate oxidative burst, producing an inve
rted-U dose response. These data indicate that J774 cells may be usefu
l for explaining some mechanisms through which the neuroendocrine syst
em interacts with the immune system.