THE PRODUCTION OF MONOCLONAL-ANTIBODY TO ANDROSTENEDIONE AND THE EFFECT OF PASSIVE-IMMUNIZATION ON XINJIANG FINE-WOOL SHEEP WITH IT

Authors
LU ZQ MA JX ZHOU CW WANG YF WANG J XIANG JY GAO Y ZENG XL
Citation
Zq. Lu et al., THE PRODUCTION OF MONOCLONAL-ANTIBODY TO ANDROSTENEDIONE AND THE EFFECT OF PASSIVE-IMMUNIZATION ON XINJIANG FINE-WOOL SHEEP WITH IT, Science in China. Series B, Chemistry, life sciences & earth sciences, 36(6), 1993, pp. 693-701
Citations number
20
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Science in China. Series B, Chemistry, life sciences & earth sciences → ACNP
ISSN journal
1001652X
Volume
36
Issue
6
Year of publication
1993
Pages
693 - 701
Database
ISI
SICI code
1001-652X(1993)36:6<693:TPOMTA>2.0.ZU;2-D
Abstract
Five hybridomas stably secreting monoclonal antibodies (McAbs) to andr ostenedione were prepared by using artificially synthesized androstene dione-11alpha-succinyl conjugate with bovine serum albumin (BSA) as an tigen. These McAbs showed slightly high cross-reactivity with testoste rone (8.1-12.3%) and estrone (0.8-2.5%) and high affinities ranging fr om 2.0 X 10(7) to 2.8 X 10(8) L/M. They were all of the IgG1 subclass. Xinjiang fine-wool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these chan ges led to increased ovulation rate and twin lambs born in young ewes.