SYNTHETIC BOUNDARY CELL IN AN ANALYTICAL ULTRACENTRIFUGE TO DETERMINETHE FLOTATION COEFFICIENT OF HUMAN SERUM-LIPOPROTEINS

Human serum lipoproteins were analyzed by a new modified synthetic bou ndary cell using an analytical ultracentrifuge. This cell allows the f ormation of the synthetic boundary at the bottom level of the cell wit h self-adjusting meniscus and baseline. Thus, the total amount of lipo proteins was seen as a single peak at first. During centrifugation, ea ch component of the lipoproteins was separated according to its flotat ion characteristics. It was, therefore, possible to determine precisel y all lipoprotein components, especially high-density lipoproteins, in the presence of a more rapidly migrating species and to calculate the flotation coefficient of each lipoprotein using the formula for sedim entation coefficient reported by Svedberg (Svedberg, T. (1925) Kolloid -Z. 36, 53-64.