CLONING AND SEQUENCE-ANALYSIS OF AN ESTERASE GENE FROM PSEUDOMONAS SPKWI-56

Authors
SHIMADA Y NAGAO T SUGIHARA A IIZUMI T YUI T NAKAMURA K FUKASE T TOMINAGA Y
Citation
Y. Shimada et al., CLONING AND SEQUENCE-ANALYSIS OF AN ESTERASE GENE FROM PSEUDOMONAS SPKWI-56, Biochimica et biophysica acta, 1174(1), 1993, pp. 79-82
Citations number
22
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1174
Issue
1
Year of publication
1993
Pages
79 - 82
Database
ISI
SICI code
0006-3002(1993)1174:1<79:CASOAE>2.0.ZU;2-F
Abstract
The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned a nd sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular wei ght agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes sugges ted that Ser-92 and His-241 might be included in the catalytic triad.