A. Flynn et al., THE ROLE OF THE BETA-SUBUNIT OF INITIATION FACTOR-EIF-2 IN INITIATIONCOMPLEX-FORMATION, Biochimica et biophysica acta, 1174(1), 1993, pp. 117-121
The functional properties of preparations of protein synthesis initiat
ion factor eIF-2 which lack the beta-subunit (as confirmed immunologic
ally) were compared with those of the heterotrimeric factor. The forme
r can bind guanine nucleotides but not initiator tRNA, and also exhibi
ts a substantially reduced rate of initiation factor eIF-2B-mediated G
DP/GTP-exchange.