Dj. Beuckelmann et al., ALTERATIONS OF K-FAILURE( CURRENTS IN ISOLATED HUMAN VENTRICULAR MYOCYTES FROM PATIENTS WITH TERMINAL HEART), Circulation research, 73(2), 1993, pp. 379-385
Prolongation of the action potential has been postulated to be a major
reason for the altered diastolic relaxation of the heart in patients
with severe heart failure. To investigate the electrophysiological bas
is for this action potential prolongation in terminal heart failure, K
+ currents were recorded in single ventricular myocytes isolated from
16 explanted hearts of patients undergoing transplantation. Results fr
om diseased hearts were compared with ventricular cells isolated from
six undiseased donor hearts. Action potential duration was significant
ly prolonged in cells from patients with heart failure. A delayed rect
ifier K+ current was hardly detectable in most cells, and if it could
be recorded, it was very small in both diseased and undiseased cells.
When currents were normalized for cell surface area, the average curre
nt density of the inward rectifier K+ current was significantly reduce
d in diseased cells when compared with normal control cells (hyperpola
rization at -100 mV, -15.9+/-2.2 vs -9.0+/-1.2 muA/cm2; P<.01). In add
ition, a large transient outward K+ current could be recorded in human
myocytes. The average current density of the time-dependent component
of this transient outward K+ current was significantly reduced in hea
rt failure (depolarization at +40 mV, 9.1+/-1.0 vs 5.8+/-0.64 muA/cm2;
p<.01). Action potential prolongation in severe heart failure may par
tially be explained by a reduction in current densities of the inward
rectifier K+ current and of the transient outward K+ current. These al
terations may thereby have a significant effect on cardiac relaxation.