EXPRESSION OF POTATO DAHP SYNTHASE IN ESCHERICHIA-COLI

Citation
Lm. Weaver et al., EXPRESSION OF POTATO DAHP SYNTHASE IN ESCHERICHIA-COLI, Bioorganic & medicinal chemistry letters, 3(7), 1993, pp. 1421-1428
Citations number
33
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
ISSN journal
0960894X
Volume
3
Issue
7
Year of publication
1993
Pages
1421 - 1428
Database
ISI
SICI code
0960-894X(1993)3:7<1421:EOPDSI>2.0.ZU;2-Z
Abstract
Expression of a potato (Solanum tuberosum L.) cDNA encoding 3-deoxy-D- arabino-heptulosonate 7-phosphate synthase, the first enzyme of the sh ikimate pathway, complemented an Escherichia coli mutant devoid of the enzyme. While the potato cDNA encodes a protein with an amino termina l putative transit sequence for chloroplast import, immunocytochemistr y localized the E. coli synthesized potato enzyme to the bacterial cyt osol.