Bh. Junker et al., CULTIVATION OF VIRUS-ANTIGEN IN FIBROBLAST CELLS USING A GLASS-FIBER BED REACTOR, Biotechnology and bioengineering, 42(5), 1993, pp. 635-642
The anchorage-dependent cell line, MRC-5, was cultivated successfully
on glass fibers with diameters ranging from 24 to 120 mum, despite vas
t differences in substrate curvature. Multilayer cell growth was obser
ved, particularly for fiber diameters 30 mum and below, which differed
from the typical monolayer growth observed in T-flask cultivations. C
ells were maintainable at a reduced incubation temperature and were de
monstrated to support virus replication for the 21-day antigen product
ion period. Direct microscopic observation, along with indirect calcul
ations, indicated that only a small fraction (about 10%) of the total
available fiber surface area was occupied by cells. Thus, productivity
per unit surface area was replaced by productivity per unit medium vo
lume when evaluating fiber bed performance. Antigen and protein yields
, as well as nutrient uptakes, were 1.5- to 2.5-fold greater than para
llel T-flask cultures when compared on this basis. Corresponding avail
able surface area-based values were 10- to 15-fold lower for the fiber
bed reactor. The multilayer cell morphology obtained in the fiber bed
was attractive for antigen production when immobilized in a column re
actor system. (C) 1993 John Wile & Sons, Inc.