MONOCLONAL-ANTIBODIES TO RH0(D) ANTIGEN . 2. SEROLOGICAL CHARACTERIZATION OF MONOCLONAL IGG1 ANTIBODIES

Authors
OLOVNIKOVA NI BELKINA EV LEMENEVA LN BERKOVSKY AL DERYUGINA EI CHERTKOV IL
Citation
Ni. Olovnikova et al., MONOCLONAL-ANTIBODIES TO RH0(D) ANTIGEN . 2. SEROLOGICAL CHARACTERIZATION OF MONOCLONAL IGG1 ANTIBODIES, Gematologia i transfuziologia, 37(9-10), 1992, pp. 5-9
Citations number
NO
Categorie Soggetti
Hematology
Journal title
Gematologia i transfuziologia → ACNP
ISSN journal
02345730
Volume
37
Issue
9-10
Year of publication
1992
Pages
5 - 9
Database
ISI
SICI code
0234-5730(1992)37:9-10<5:MTRA.2>2.0.ZU;2-A
Abstract
Four IgG1 monoclonal antibodies (MoAbs) to the Rh antigen D, produced by stable Epstein-Barr virus transformed B-lymphoblastoid cell lines w ere assessed in different serological tests. Their suitability in bloo d group typing was shown in antiglobulin, enzyme or albumin methods. T he specificity and activity of MoAbs was tested with a panel of red ce lls of various Rh-phenotypes. The supernatants of all four showed anti -D specificity and ability to react with D(u) red cells. Mean level of MoAbs concentration was near 10 mug/ml These human anti-D MoAbs prove d to be useful D-typing diagnostic reagents.