SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF HI GH-MOLECULAR-WEIGHT DNAS

Authors
YAMAGUCHI M IIZUMI T YAMAMOTO T MOTOZATO Y
Citation
M. Yamaguchi et al., SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF HI GH-MOLECULAR-WEIGHT DNAS, Nippon kagaku kaishi, (7), 1993, pp. 837-844
Citations number
4
Categorie Soggetti
Chemistry
Journal title
Nippon kagaku kaishi → ACNP
ISSN journal
03694577
Issue
7
Year of publication
1993
Pages
837 - 844
Database
ISI
SICI code
0369-4577(1993):7<837:SC(OHG>2.0.ZU;2-Y
Abstract
Size-exclusion chromatography (SEC) of DNAs was investigated with stai nless steel columns (6 mml.D. x 250 mm) packed with macroporous spheri cal gels prepared from glucomannan. High molecular weight DNAs (>15kb) were eluted following size-exclusion theory from the columns packed w ith large gels (>130 mum). However, the smaller gels resulted in the l arger elution times for those DNAs. The particle size as well as the p ore size of the packing material were found to be important factors fo r chromatographic separation of high molecular weight DNAs.