SIZE-EXCLUSION CHROMATOGRAPHIC CHARACTERIZATION OF SODIUM HYALURONATEFRACTIONS PREPARED BY HIGH ENERGETIC SONICATION

Authors
ORVISKY E SOLTES L CHABRECEK P NOVAK I STANCIKOVA M
Citation
E. Orvisky et al., SIZE-EXCLUSION CHROMATOGRAPHIC CHARACTERIZATION OF SODIUM HYALURONATEFRACTIONS PREPARED BY HIGH ENERGETIC SONICATION, Chromatographia, 37(1-2), 1993, pp. 20-22
Citations number
28
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
37
Issue
1-2
Year of publication
1993
Pages
20 - 22
Database
ISI
SICI code
0009-5893(1993)37:1-2<20:SCCOSH>2.0.ZU;2-X
Abstract
A high molecular weight sodium hyaluronate isolated from rooster combs was degraded by ultrasonication. High-performance size exclusion chro matography allowed rapid and accurate determination of molecular weigh t parameters (M(z), M(w), M(n)) and distributions. The time dependence of hyaluronan ultrasonication to the molecular characteristics of the polymer was investigated. A non-random nature of the degradation proc ess was demonstrated and the reciprocal Mn value was found to be linea rly proportional to the time of ultrasonication.