The bioleaching of a low-grade uranium ore in shaker and columns using
natural, pure and mixed cultures has been studied. Initially, a chemi
cal and microscopical characterization of the mineral was carried out.
Orbital shaker experiments were performed to quickly obtain the best
bacterial leaching conditions. Afterwards, small columns were used to
determine other variables related to percolation leaching. Finally, an
uranium ore from the Fe mine (Ciudad Rodrigo, Spain) was leached in t
he presence of bacteria using large columns (24 cm diameter and 275 cm
height). The most important results were: (i) the ore contains suffic
ient pyrite in order for uranium bioleaching to take place under optim
um conditions; (ii) shaker experiments showed that temperature, pH and
type of inoculum are the most important variables in bioleaching; (ii
i) two different ores were attacked in the columns: altered and non-al
tered. In the first case, the extraction rate of uranium was higher. H
owever, in both cases the final efficiency was very similar (95%).