CLONING AND NUCLEOTIDE-SEQUENCE ANALYSIS OF THE CANDIDA-ALBICANS ENOLASE GENE

The complete nucleotide sequence of the coding region as well as the f lanking non-coding region of Candida albicans enolase gene was determi ned. A continuous open reading frame of 1323 nucleotides with no intro ns was identified. The deduced amino acid sequence showed 87% similari ty to the enolases from the yeast Saccharomyces cerevisiae. The two is oforms of enolase are encoded by two non-tandemly arrayed genes in S. cerevisiae. However, DNA hybridisation analysis indicates that in C. a lbicans enolase is encoded by a single gene. The position of the trans cription start site, putative TATA box and polyadenylation signal of t he C. albicans enolase gene have been identified. The location of thes e sequences are similar to those of the S. cerevisiae enolase genes.