EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN A NEURONAL CELL-LINE - FUNCTIONAL-ACTIVITY OF PROXIMAL REGULATORY ELEMENTS

To study cell type-specific regulation of the gene for amyloid precurs or protein (APP), we have analysed the human APP promoter for transcri ptional activity and interaction with nuclear proteins in the neuronal cell line NG108-15. Sequences from - 203 to + 104 were sufficient for promoter function and regulatory elements within this region (- 128 t o - 63) were identified by DNase I protection experiments. These resul ts suggest that essentially the same proximal elements are recognized by nuclear factors from both neuronal and nonneuronal cells.