HORSERADISH PEROXIDASE-LABELED OLIGONUCLEOTIDES AND FLUORESCENT TYRAMIDES FOR RAPID DETECTION OF CHROMOSOME-SPECIFIC REPEAT SEQUENCES

We present a sensitive and rapid fluorescence in situ hybridization (F ISH) strategy for detecting chromosome-specific repeal sequences. It u ses horseradish peroxidase (HRP)-labeled oligonucleotide sequences in combination with fluorescent tyramide-based detection. After in situ h ybridization, the HRP conjugated to the oligonucleotide probe is used to deposit fluorescently labeled tyramide molecules at the site of hyb ridization. The method features full chemical synthesis of probes, str ong FISH signals, and short processing periods: as well as multicolor capabilities.