GENERATION AND PARTIAL CHARACTERIZATION OF AN EOSINOPHIL CHEMOTACTIC CYTOKINE PRODUCED BY SENSITIZED EQUINE MONONUCLEAR-CELLS STIMULATED WITH STRONGYLUS-VULGARIS ANTIGEN
Va. Dennis et al., GENERATION AND PARTIAL CHARACTERIZATION OF AN EOSINOPHIL CHEMOTACTIC CYTOKINE PRODUCED BY SENSITIZED EQUINE MONONUCLEAR-CELLS STIMULATED WITH STRONGYLUS-VULGARIS ANTIGEN, Veterinary immunology and immunopathology, 37(2), 1993, pp. 135-149
Supernatants generated by stimulation of peripheral blood mononuclear
cells (PBMC) from Strongylus vulgaris sensitized or immunized ponies w
ere assayed in vitro for eosinophil chemotactic activity (ECA) using t
he filter system in blind well chambers. The supernatants from these c
ultures were chemotactic for eosinophils, but not for neutrophils. Sup
ernates from cultures of unsensitized PBMC stimulated with S. vulgaris
antigen were not chemotactic for eosinophils. ECA was first detected
in culture supernatants after 1.5 h of incubation and was dependent on
both antigen and PBMC concentrations, but independent of serum concen
trations. Both female and male S. vulgaris worm antigens stimulated EC
A production from sensitized PBMC. ECA was not induced by in vitro sti
mulation of sensitized S. vulgaris PBMC by female Strongylus edentatus
worm antigen. Partial characterization of the eosinophil chemotactic
cytokine showed it to be nondialyzable, greater than 8000 molecular we
ight (MW), and sensitive to heating (56 and 95-degrees-C), trypsin, an
d sodium metaperiodate treatments, suggesting that the cytokine is a p
rotein containing some essential carbohydrate moieties. The cytokine d
escribed in this paper could partially contribute to the in vivo blood
and tissue eosinophilia in experimental S. vulgaris infection.