IN-VITRO FERTILIZATION OF PIG OVA - EFFECTS OF VARIOUS FACTORS ON PENETRATION, POLYSPERMY AND MALE PRONUCLEUS DEVELOPMENT

Citation
E. Behalova et al., IN-VITRO FERTILIZATION OF PIG OVA - EFFECTS OF VARIOUS FACTORS ON PENETRATION, POLYSPERMY AND MALE PRONUCLEUS DEVELOPMENT, Animal reproduction science, 32(1-2), 1993, pp. 127-133
Citations number
22
Categorie Soggetti
Reproductive Biology","Veterinary Sciences
Journal title
ISSN journal
03784320
Volume
32
Issue
1-2
Year of publication
1993
Pages
127 - 133
Database
ISI
SICI code
0378-4320(1993)32:1-2<127:IFOPO->2.0.ZU;2-9
Abstract
Pig zona-free and zona-intact ova were successfully penetrated in vitr o by ejaculated boar spermatozoa. The oocytes were obtained from antra l follicles (2-5 mm in diameter) of slaughtered gilts and matured in M -1 99 with porcine follicular fluid (50%) and follicle stimulating hor mone (FSH) (0.25 IU ml-1) for 44 h at 38.5-degrees-C. In vitro fertili sation (IVF) was carried out with a spermatozoa-rich fraction of a fre sh boar ejaculate which had been incubated in M-199 supplemented by 10 % inactivated bovine serum. The penetration rate of zona-free oocytes was dependent on the duration of sperm incubation. IVF of zona-intact ova was designed to estimate the optimal spermatozoa concentration for insemination. The concentration of (1-1.5) X 10(6) spermatozoa ml-1 g ave the best fertilisation results (87%), whereas the concentration be low 1 X 10(6) spermatozoa ml-1 resulted in a lower fertilisation rate (62%) but in a higher number of monospermic eggs (36%) and male pronuc leus formation (80%).