Hm. Elberry et al., REGULATION OF THE UREA ACTIVE TRANSPORTER GENE (DUR3) IN SACCHAROMYCES-CEREVISIAE, Journal of bacteriology, 175(15), 1993, pp. 4688-4698
The DUR3 gene, which encodes a component required for active transport
of urea in Saccharomyces cerevisiae, has been isolated, and its seque
nce has been determined. The deduced DUR3 protein profile possesses al
ternating hydrophobic and hydrophilic regions characteristic of integr
al membrane proteins. Strong negative complementation observed during
genetic analysis of the DUR3 locus suggests that the DUR3 product may
polymerize to carry out its physiological function. Expression of DUR3
is regulated in a manner similar to that of other genes in the allant
oin pathway. High-level expression is inducer dependent, requiring fun
ctional DAL81 and DAL82 genes. Maintenance of DUR3 mRNA at uninduced,
nonrepressed basal levels requires the negatively acting DAL80 gene pr
oduct. DUR3 expression is highly sensitive to nitrogen catabolite repr
ession and also has a partial requirement for the GLN3 product.