IMMUNOLOCALIZATION OF PLATELET-DERIVED GROWTH-FACTOR A-CHAIN AND B-CHAIN AND PDGF-ALPHA AND BETA-RECEPTORS IN HUMAN GINGIVAL WOUNDS

Citation
Rj. Green et al., IMMUNOLOCALIZATION OF PLATELET-DERIVED GROWTH-FACTOR A-CHAIN AND B-CHAIN AND PDGF-ALPHA AND BETA-RECEPTORS IN HUMAN GINGIVAL WOUNDS, Journal of Periodontal Research, 32(2), 1997, pp. 209-214
Citations number
25
Categorie Soggetti
Dentistry,Oral Surgery & Medicine
ISSN journal
00223484
Volume
32
Issue
2
Year of publication
1997
Pages
209 - 214
Database
ISI
SICI code
0022-3484(1997)32:2<209:IOPGAA>2.0.ZU;2-R
Abstract
Platelet-derived growth factor (PDGF) is a polypeptide growth factor w hich has been implicated as a major mitogen involved in wound healing. The PDGF appears to promote periodontal regeneration; however, its di stribution in gingival tissues is not known and how it participates in gingival wound healing is unclear. Using highly specific antibodies w e have studied the distribution of PDGF A and B chains and alpha- and beta-PDGF receptors in healing human gingival wounds. Wounds were crea ted by making a 0.75 mm deep incision in the papilla of healthy human gingiva and biopsies were obtained from the same site after 8 h and 1, 3, 7, 14 and 21 d. Frozen sections were immunostained with affinity p urified antibodies. The results showed that both epithelium and fibrin clot manifested positive immunostaining for anti-PDGF-A and B-chain a ntibodies. Staining was present in unwounded and wounded epithelia, an d in the fibrin clot it appeared to be more intense for the PDGF-A cha in. Blood vessels in connective tissue were also positive while other areas were largely negative. No significant staining was detectable in healthy tissues for anti-PDGF-alpha or -beta receptor antibodies. How ever, the wound site began to manifest positive immunostaining for ant i beta-receptor antibody after 3 d of healing, became maximal at 7 d, and then decreased. Our data indicate, but do not prove, that gingival epithelium may be a source of PDGF A and B chains and that the A chai n may have a more prominent role to play during early stages of healin g. Expression of PDGF beta-receptor appears later at the wound site, i ndicating that the PDGF B isomer may regulate later wound healing even ts.