R. Entzeroth et al., EVIDENCE OF SECRETION OF AN INTERNAL ANTIGEN OF EIMERIA-PAPILLATA INTO CULTURED-CELLS, Archiv fur Protistenkunde, 143(4), 1993, pp. 347-351
Monoclonal antibodies (MAB) were raised against whole sporozoites of E
imeria papillata and their organelle fractions. These fractions includ
ed rhoptries, dense granules, mitochondria and pellicle fragments. Twe
nty MAB recognized structures within the apical region of sporozoites
as observed by indirect immunofluorescence antibody (IFA) test. Of the
se, 2 MAB showed a reaction with extracellular and intracellular sporo
zoites at the apical region as well as with after structures within in
vaded cells in culture (24 hr monolayers of MDCK cells). Within 60 min
utes of inoculation fluorescence in the intracellular sporozoites decr
eased. This decrease corresponded with IFA reactions in the invaded ce
lls in the form of cytoplasmic granules, on the host cell surface and
in the vicinity of the parasitophorous vacuole. These results indicate
that proteins of parasite origin are released from the apical region
and are dispersed in different parts of the host cell.