PRELIMINARY IMMUNOHISTOCHEMICAL NEW FINDINGS IN THE MYENTERIC PLEXUS OF PATIENTS WITH INTESTINAL NEURONAL DYSPLASIA TYPE-B

Citation
K. Munakata et al., PRELIMINARY IMMUNOHISTOCHEMICAL NEW FINDINGS IN THE MYENTERIC PLEXUS OF PATIENTS WITH INTESTINAL NEURONAL DYSPLASIA TYPE-B, European journal of pediatric surgery, 7(1), 1997, pp. 21-29
Citations number
22
Categorie Soggetti
Surgery,Pediatrics
ISSN journal
09397248
Volume
7
Issue
1
Year of publication
1997
Pages
21 - 29
Database
ISI
SICI code
0939-7248(1997)7:1<21:PINFIT>2.0.ZU;2-R
Abstract
In order to investigate the causes of abnormal peristalsis of the colo n in intestinal neuronal dysplasia (IND), we studied the structure of the myenteric plexus of IND colon using silver-impregnation (Suzuki's method) as well as the innervation of both IND colons and normal colon s using immunofluorescence technique with monoclonal antibodies to syn aptic vesicles, and antisera to vasoactive intestinal polypeptide (VIP ), substance P (SP), methionine-enkephalin (Met-Enk), and gastrin-rele asing peptide (CRP). The following results were obtained. 1) In the IN D colon, the number of identifiable myenteric ganglia was decreased. I n a few cases of IND, irreversible neuron degeneration can be involved in the pathogenesis of IND. 2) In the IND colon, the distribution and fluorescence intensity of synaptic vesicles coincided with those of p eptidergic nerve fibers. In the normal colon, synaptic vesicles were m uch more numerous in the circular muscle layers than in the longitudin al muscle layers, and the fluorescence intensity of those in the circu lar muscle layers was stronger than that of those in the longitudinal muscle layers. On the other hand, in IND colon, there were fewer synap tic vesicles in the circular muscle layers, and their fluorescence int ensity was weak, while there were many synaptic vesicles in the longit udinal muscle layers, and their fluorescence intensity was strong. 3) Morphological abnormalities may exist in synaptic vesicles in the circ ular muscle layers of the IND colon. 4) Regarding the peptidergic nerv e fibers, in the IND colon, innervation of circular muscle layers by M et-Enk-, GRP- and SP-immunoreactive fibers was reduced, and longitudin al muscles were more strongly innervated by immunoreactive fibers than those in the normal colon. 5) Disturbed innervation of non-adrenergic non-cholinergic excitatory nerves may cause the disturbance of muscle contractions in the IND colon. In addition, an imbalance of peptiderg ic and synaptic vesicle's innervations in both muscle layers may be re lated to the abnormal peristalsis of IND colon. Also, morphological ab normalities of synaptic vesicles may be concerned with its abnormal pe ristalsis.