Kd. Rane et Ka. Sims, PRODUCTION OF CITRIC-ACID BY CANDIDA-LIPOLYTICA-Y1095 - EFFECT OF GLUCOSE-CONCENTRATION ON YIELD AND PRODUCTIVITY, Enzyme and microbial technology, 15(8), 1993, pp. 646-651
The effects of temperature and initial pH on growth of Candida guillie
rmondii Y 488, Candida lipolytica Y 1095, Yarrowia lipolytica Y 7576,
and Saccharomycopsis lipolytica DRL 99 were evaluated. All four yeasts
exhibited maximum biomass production at 27-degrees-C. Except for S. l
ipolytica DRL 99, increased agitation nearly doubled the amount of bio
mass produced at 27-degrees-C. The growth of S. lipolytica DRL 99 was
also affected by initial medium pH. Based on the results of these expe
riments, C. lipolytica Y 1095 was selected for citric acid production
studies in a 2-l batch fermentor. At a glucose concentration of 3% w/v
, the maximum specific growth rate of C. lipolytica Y 1095 was 0.38 h-
1. During the production phase, citric acid yields ranged from 0.50 to
0.79 g citric acid produced per gram glucose consumed and the volumet
ric productivity ranged from 0.27 to 0.60 g citric acid per liter per
hour. Final citric acid concentrations ranged from 13.6 to 78.5 g citr
ic acid per liter and were dependent on the initial glucose concentrat
ion in the production medium.