T. Vares et al., PRODUCTION OF MULTIPLE LIGNIN PEROXIDASES BY THE WHITE-ROT FUNGUS PHLEBIA-OCHRACEOFULVA, Enzyme and microbial technology, 15(8), 1993, pp. 664-669
The white-rot fungus Phlebia ochraceofulva (Bourd. & Galz.) Donk. stra
in 75144 actively produced lignin peroxidase (LiP) under nitrogen-limi
ted cultivation conditions. Maximal activity was reached during days 6
-8 of growth in bioreactors containing immobilized mycelium and either
a low basal concentration of Mn(II) (0.12 ppm) or a 10-fold concentra
tion of Mn(II) (1.2 ppm). Laccase activity was also detected in the ex
tracellular fluid of P. ochraceofulva, but no Mn-peroxidase (MnP) acti
vity was observed with either level of Mn(II). Five lignin peroxidases
were purified and partially characterized.