S. Ting, LIQUID-CHROMATOGRAPHIC DETERMINATION OF SCOPOLAMINE, HYOSCYAMINE, ANDPHENOBARBITAL IN TABLETS, Journal of AOAC International, 80(2), 1997, pp. 331-333
A liquid chromatographic method using a reversed-phase C-18 column and
octanesulfonic acid sodium salt-methanol as the mobile phase was deve
loped for the simultaneous determination of phenobarbital, scopolamine
, and hyoscyamine in tablets, The mixture of the 3 drugs was resolved
in <8 min, Detector responses were linear for 10 mu L injections of th
e following: scopolamine hydrobromide, 8.25-206.3 mu g/mL; hyoscyamine
sulfate, 15.01-750.76 mu g/mL; and phenobarbital, 250-751 mu g/mL, Re
coveries from tablets were 100.8% for scopolamine hydrobromide, 100.1%
for hyoscyamine sulfate, and 100.3% for phenobarbital. Replicate inje
ctions of scopolamine hydrobromide, hyoscyamine sulfate, and phenobarb
ital gave an overall relative standard deviation of <1.0% (n = 10), Th
e method detected as little as 3.3 ng scopolamine hydrobromide.