APPLICATION OF AN INTEGRATED RATE-EQUATION TO THE INACTIVATION OF CATALASE

A feature of catalase that has received scant attention in recent year s and that may have physiological significance is the peroxide-depende nt inactivation of the enzyme. In this article we show how to obtain t he second-order rate constant for the inactivation reaction by fitting the reaction progress curve to an integrated rate equation. This meth od will simplify quantitation of the inactivation and reactivation of catalase. These measurements in tissues with altered metabolic states may reveal new information about the role of catalase in nutrition, ag ing, and pathology.