The B- and T-cell antigenic sites on type 0 pili from Pseudomonas aeru
ginosa were determined by using an antipilus antibody competition assa
y and PAO-immune T-cell blasts in proliferation studies. The citracony
lated tryptic digest III region (residues 54 to 120) was determined to
be an immunodominant site for both T and B cells on the pilin molecul
e.