IDENTIFICATION OF THE REPLICATION REGION OF THE LACTOBACILLUS-ACIDOPHILUS PLASMID PLA106

Authors
SANO K OTANI M OKADA Y KAWAMURA R UMESAKI M OHI Y UMEZAWA C KANATANI K
Citation
K. Sano et al., IDENTIFICATION OF THE REPLICATION REGION OF THE LACTOBACILLUS-ACIDOPHILUS PLASMID PLA106, FEMS microbiology letters, 148(2), 1997, pp. 223-226
Citations number
15
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
148
Issue
2
Year of publication
1997
Pages
223 - 226
Database
ISI
SICI code
0378-1097(1997)148:2<223:IOTRRO>2.0.ZU;2-W
Abstract
The complete nucleotide sequence of plasmid pLA106 (2862 p) from Lacto bacillus acidiophilus TK8912 was determined. Based on this sequence, t he location of the genes for mobilization-plasmid recombination protei n (mob), replication origin (ori), transcriptional repressor protein ( repA) and replication initiation protein (repB) were predicted. Deleti on analysis showed that the 1.4-kb PstI-EcoRV fragment carrying the or i, repA and repB genes is able to replicate in Lacrobacillus and Esche richia coil cells. The plasmid pLA106 appears to have most features of the pLS1/pE194 plasmid family.