CLONAL RELATIONSHIPS AMONG ESCHERICHIA-COLI SEROGROUP O6 ISOLATES BASED ON RAPD

Authors
PACHECO ABF GUTH BEC SOARES KCC DEALMEIDA DF FERREIRA LCS
Citation
Abf. Pacheco et al., CLONAL RELATIONSHIPS AMONG ESCHERICHIA-COLI SEROGROUP O6 ISOLATES BASED ON RAPD, FEMS microbiology letters, 148(2), 1997, pp. 255-260
Citations number
18
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
148
Issue
2
Year of publication
1997
Pages
255 - 260
Database
ISI
SICI code
0378-1097(1997)148:2<255:CRAESO>2.0.ZU;2-1
Abstract
The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotyp es H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. The amplified band arrays allo wed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains coul d be assigned to either H1 or H31 serotypes. The results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clo nal markers in this E. coli group.