H. Kumazawa et al., INFLUENCE OF LAK CELLS ON EXPRESSION OF HLA-DR ANTIGEN ON LARYNGEAL CARCINOMA CELL-LINE IN NEW CULTURE SYSTEMS, European archives of oto-rhino-laryngology, 250(4), 1993, pp. 224-228
We demonstrated the enhancement of HLA-DR antigen expression on cultur
ed laryngeal carcinoma cells (Hep 2) by in vitro cultivation with LAK
cells using flow cytometric and immunohistological analysis. For in vi
tro cultivation of tumor cells with LAK cells, we used newly developed
experimental systems (the Transwell double-dish system and experiment
al three-dimensional tumors). In flow cytometric analysis, expression
of HLA-DR antigen on tumor cells was compared before and after co-cult
ivation with LAK cells. When tumor cells were cultured separately with
LAK cells in a Transwell Petri dish and the expression of HLA-DR anti
gen on tumor cells was analyzed by flow cytometry, the expression of H
LA-DR antigen on tumor cells was increased in a dose-dependent manner
related to the number of LAK cells used. Furthermore, when anti-interf
eron-gamma monoclonal antibody was added to the experimental system, e
nhancement of HLA-DR antigen expression was blocked. These findings we
re consistent with immunohistological studies, in which experimental t
hree-dimensional Hep 2 cell tumors were established in double-layered
agar with/without being co-cultivated with LAK cells. The expression o
f HLA-DR antigen in this system was significantly increased when compa
red to such expression before cultivation with LAK cells. These findin
gs suggested that the culture systems employed in this study could be
a possible model for examining solid tumor in vivo biological response
s. This enhanced expression of HLA-DR antigen may also represent one o
f the multifactorial responses seen with adoptive LAK cell immunothera
py for solid tumors.