CHRONIC TREATMENT WITH ANTI-ENDOTHELIN ANTIBODIES FAILS TO MODIFY THEDEVELOPMENT OF HYPERTENSION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS AND DOCA-SALT HYPERTENSIVE RATS
M. Yasujima et al., CHRONIC TREATMENT WITH ANTI-ENDOTHELIN ANTIBODIES FAILS TO MODIFY THEDEVELOPMENT OF HYPERTENSION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS AND DOCA-SALT HYPERTENSIVE RATS, Tohoku Journal of Experimental Medicine, 169(1), 1993, pp. 43-50
This study was designed to assess whether blocking endogenous endothel
in with anti-endothelin antibodies could alter the development of hype
rtension in stroke-prone spontaneously hypertensive rats (SHR) and DOC
A-salt treated rats. Specific anti-endothelin antibodies were produced
in rabbits by standard methods. The amount of anti-endothelin antibod
ies employed in this study blocked the hypertensive effect of endothel
in-1, 750 ng/kg, by 55% in conscious rats. Intravenous injection of an
ti-endothelin antibodies as a bolus twice a week for 3 weeks did not a
ffect the rise in blood pressure of stroke-prone SHR (268 +/- 8 mmHg,
n = 8) compared to control stroke-prone SHR (256 +/- 7 mmHg, n = 8) tr
eated with normal rabbit serum. Intravenous administration of anti-end
othelin antibodies in a same manner also failed to alter the developme
nt of hypertension in DOCA-salt treated rats (160 +/- 6 mmHg in anti-e
ndothelin antibodies-treated group, n = 7 compared to 164 +/- 5 mmHg i
n normal rabbit serum-treated group, n = 7). The administration of ant
i-endothelin antibodies did not induce any significant changes in body
weight, urine volume and urinary sodium excretion in stroke-prone SHR
and DOCA-salt treated rats compared to those treated with normal rabb
it serum. These findings suggest that circulating endothelin might not
play a major role in the regulation of blood pressure in stroke-prone
SHR and DOCA-salt treated rats.