LIBERATION OF PROSTAGLANDINS AND LEUKOTRI ENES FROM SYNOVIAL TISSUE, CARTILAGE AND BONES UNDER DICLOFENAC THERAPY

The prostaglandin (PG) E2-, 6-keto-PGF1alpha- and and leukotriene (LT) C4- release from synovial tissue, cartilage, corticalis and cancellou s bone was determined in patients with osteoarthritis of the knee join t. Before surgery the patients received diclofenac in three applicatio n modes (oral, rectal, local). The tissue was incubated directly, afte r washing with a buffer solution and after in vitro administratin of d iclofenac to the washed tissue. A significant decrease in PG synthesis inhibition in control samples in comparison to the direct incubated s amples was found for PGE2 after rectal, for 6-keto-PGF1alpha after rec tal and oral medication in synovialis. In cartilage and bone tissue th ere was only a significant increase of the PGE2- SyntheSis inhibition for corticalis after rectal application. After in vitro application of diclofenac there was a significant inhibition of the prostaglandin sy nthesis. The LT release from synovial tissue was increased after washi ng in the group with local application significantly, in both other gr oups not significantly. The in-vitro application of diclofenac showed no effect on LT synthesis. Diclofenac concentration after oral or rect al application in synovial tissue was 3.9 or 2.6, in cartilage 1.4 or 0.7 and bone 1.1 or 1.91 X 10(-7) mol/l.