N. Foisset et al., SEGREGATION ANALYSIS OF ISOZYME MARKERS ON ISOLATED MICROSPORE-DERIVED EMBRYOS IN BRASSICA-NAPUS L, Plant breeding, 110(4), 1993, pp. 315-322
The segregation of isozyme genetic markers was studied on embryos aris
ing from isolated microspore cultures in five rapeseed F1 hybrid genot
ypes. Out of the ten isozyme genes considered, five (Aco-1, Aco-3, Lap
-1, Pgm-3, Tpi-1) did not segregate according to expected Mendelian ra
tios in at least two Fl crosses. F2 plants, generated from the same hy
brids, included in the analysis, were free of segregation distortions.
Linkage analysis between each segregating enzyme locus in each progen
y revealed independence between these markers. In this paper, the resu
lts of the linkage analysis as well as the origins of the distortions
are discussed. The existence of androgenic embryogenesis genes may be
responsible for the abnormal segregations of the five isozyme genes.