MSR - A PROGRAM FOR KINETIC-ANALYSIS OF ENZYME MODIFICATION DATA ON PC

A computer program has been written for the kinetic method of substrat e reaction (MSR) in enzyme modification studies. The program analyses a series of progress curves that continuously record signals (absorban ce, fluorescence emission, etc.) reflecting the concentration changes of substrate or product during enzyme modification in the presence of substrate. After the apparent kinetic parameters are calculated by gra phdrawing or curve-fitting method, a series of relevant plots is shown , from which information about the enzyme-inhibitor interaction can be deduced and microscopic kinetic constants calculated.