SCHIZODEME AND ZYMODEME ANALYSIS OF TRYPANOSOMES OF THE SUBGENUS SCHIZOTRYPANUM FROM THE BAT

Two stocks and nine clones of trypanosomes of the subgenus Schizotrypa num were analyzed based on their electrophoretic pattern of EcoR1 dige stion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzymes (zymodeme). The trypanosome stocks were isolated from Phyl lostomus hastatus bats collected in different locations in Minas Gerai s State, Brazil. The k-DNA pattern showed major variations between sto cks and microvariations among the clones. Furthermore, in one of sever al cloning attempts, two different populations could be isolated. Amon g the six enzymes studied, glucose phosphate isomerase presented diffe rent patterns for stocks and clones. No similarity was found among bat trypanosomes and Trypanosoma (Schizotrypanum) cruzi standard zymodeme s. This shows that bat trypanosomes are distinct from T. (S.) cruzi.