E. Pissiotis et L. Spangberg, THE IMPORTANCE OF THE CULTURE-MEDIUM ON HUMAN DENTAL-PULP CELL ATTACHMENT, Archives of oral biology, 38(8), 1993, pp. 641-647
Radiochromium-labelled pulpal cells were cultured with nine commercial
ly available media and conditioned minimal essential (MEM) medium. Rad
iochromium release from the 10 cell cultures was recorded after 4 h of
incubation. Cells cultured with MEM and conditioned MEM were examined
by scanning electron microscopy (SEM) after 2, 4, 8 and 24 h of incub
ation to study possible differences in attachment. Radiochromium relea
se from cells cultured with most of the media was very high, indicatin
g damage to the external cell membrane. When cells were cultured with
conditioned medium, the radiochromium release was within acceptable li
mits. SEM showed faster attachment when cells were cultured with condi
tioned MEM. The data indicate that pulpal cells should be cultured wit
h conditioned medium for better results in short-term cytotoxicity exp
eriments with the radiochromium release method.