2-SITE ELISA FOR QUANTIFICATION OF THE TERMINAL C5B-9 COMPLEMENT COMPLEX IN PLASMA - USE OF MONOCLONAL AND POLYCLONAL ANTIBODIES AGAINST A NEOANTIGEN OF THE COMPLEX

Authors
ACCARDOPALUMBO A TRIOLO G CASIGLIA D SALLI L GIARDINA E TRIOLO G
Citation
A. Accardopalumbo et al., 2-SITE ELISA FOR QUANTIFICATION OF THE TERMINAL C5B-9 COMPLEMENT COMPLEX IN PLASMA - USE OF MONOCLONAL AND POLYCLONAL ANTIBODIES AGAINST A NEOANTIGEN OF THE COMPLEX, Journal of immunological methods, 163(2), 1993, pp. 169-172
Citations number
14
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
163
Issue
2
Year of publication
1993
Pages
169 - 172
Database
ISI
SICI code
0022-1759(1993)163:2<169:2EFQOT>2.0.ZU;2-X
Abstract
A quantitative ELISA procedure using monoclonal and polyclonal antibod ies against neoantigens of the terminal C5b-9 complement complex has b een developed. The ELISA was demonstrated to be both sensitive and rep roducible. The normal range for C5b-9 determinations, defined as 2.5-9 7.5% interval of the values obtained in 76 healthy blood donors, was 3 .12-10.3 AU/ml. The presence of rheumatoid factor did not affect the d etermination of C5b-9 as demonstrated by immunoabsorption studies.