D. Patel et al., USE OF DENSITY PERTURBATION TO ISOLATE IMMUNOLOGICALLY DISTINCT POPULATIONS OF CELLS, Journal of immunological methods, 163(2), 1993, pp. 241-251
Experiments have been carried out to demonstrate that, using antibody
coated-Dynabeads as a model system for density labelling MOLT-4 T cell
s, the overall density of cells can be increased such that the cells t
hat bind particles can be separated on isopycnic isotonic density grad
ients from cells that bind fewer particles. The increase in density is
dependent on the cell volume and the number of particles bound. After
centrifugation, cells with bound particles were found at positions in
the gradient that reflected their increased density. Observed density
ranges for cells with particular numbers of particles bound coincided
closely with calculated expected density ranges. These results indica
te the potential for separation of different subpopulations of cells o
n the basis of the immunological identity of the surface of cells usin
g density perturbation methods involving antibody coated-density parti
cles.