MOLECULAR-CLONING AND EXPRESSION OF MOUSE AND HUMAN CDNA-ENCODING AESAND ESG PROTEINS WITH STRONG SIMILARITY TO DROSOPHILA ENHANCER OF SPLIT GROUCHO PROTEIN

Mouse and human cDNA encoding AES (amino-terminal enhancer of split) a nd ESG (enhancer of split groucho) proteins with strong similarity to Drosophila enhancer of split groucho protein were isolated and sequenc ed. Mouse AES-1 and AES-2 proteins, probably resulting from alternativ e splicing, contain 202 and 196 amino acids, respectively, while mouse ESG protein consists of 771 amino acids. The amino acid sequences of mouse and human AES proteins were found to exhibit approximately 50% i dentity to the amino-terminal region of Drosophila groucho, mouse ESG and human transducin-like enhancer of split (TLE) proteins. Mouse AES transcripts of 1.5 kb and 1.2 kb were abundantly expressed in muscle, heart and brain. Human AES transcripts of 1.6 kb and 1.4 kb were predo minantly present in muscle, heart and placenta. Mouse ESG (homolog of human TLE 3) transcripts of 3.3 kb and 4.0 kb were found only in testi s, while human TLE 1 transcripts of 4.5 kb was more abundant in muscle and placenta compared to heart, brain, lung, liver, kidney and pancre as. Human AES, TLE 1 and TLE 3 genes were mapped to chromosomes 19, 9 and 15, respectively, using human and Chinese hamster hybrid cell line s.