CONSTRUCTION OF NEW T-VECTORS FOR DIRECT CLONING OF PCR PRODUCTS

More than half of the products of PCR contain an extra A residue at th e 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without m odification, T vectors, which have a single overhanging T residue at t he 3' end, have been developed. In the present study, we constructed n ew T vectors which can be prepared in the laboratory by simple digesti on with the restriction enzymes AspEI or Eam1105I.