ACROSOME REACTION OF BOAR SPERMATOZOA IN HOMOLOGOUS IN-VITRO FERTILIZATION

Ejaculated spermatozoa from four different boars were used to evaluate the acrosome reaction during in vitro fertilization with homologous o vulated oocytes. The acrosome reaction was assessed according to a per oxidase-labeling peanut agglutinin method and a triple-stain technique . An increase in the proportion of living sperm with reacted acrosomes was observed after preincubation and 2 hr of coincubation (P < 0.05). The percentage of true acrosome-reacted sperm remained reasonably con stant throughout coincubation. In vitro penetration rates of oocytes v aried among boars, but no relationship was found between fertilization rates of oocytes and maximum percentages of acrosome reacted living s perm. (C) 1993 Wiley-Liss, Inc.