INVESTIGATION ON THE ELECTROPHORETIC BEHA VIOR OF DIPEPTIDES IN HIGH-PERFORMANCE CAPILLARY ZONE ELECTROPHORESIS

In this paper, the factors influencing the migration reproducibility o f dipeptides in HPCZE were discussed. Two migration parameters-migrati on time and relative migration time were investigated as the functions of operation current and column temperature. The relative migration t ime was proposed to be an index for peak identification in HPCZE. The effects of applied current and column temperature on migra tion time h ave been discussed. The linear correlation between lg t(m) and T-1 has been derived and has been explained based on the fundamental migratio n equation in HPCZE. The criteria of relative migration time which are independent of applied current and column temperature shows the chara cteristic constant for a specific column in HPCZE.