HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL CONCENTRATIONS AND POSTHEPARIN HEPATIC AND LIPOPROTEIN LIPASES IN OBESITY - RELATIONSHIPS WITH PLASMA-INSULIN LEVELS

The concentration of high-density lipoprotein (HDL) cholesterol in pat ients with severe obesity is generally subnormal. The exact mechanism linking obesity with reduced levels of HDL cholesterol remains unclear . In this study we evaluated the postheparin plasma lipolytic enzymes lipoprotein lipase (LPL) and hepatic lipase (HL) in a group of 24 obes e women compared with controls and analyzed the interrelationships bet ween insulin, postheparin lipolytic enzymes and HDL subfractions. Tota l HDL cholesterol was significantly lower in the obese subjects than i n the controls, and the difference was mainly due to HDL2 cholesterol concentrations. Mean fasting glucose, insulin and the summated means o f glucose (SIGMA glucose) after the oral glucose tolerance test (OGTT) were not significantly different in the two groups. The summated mean s of insulin (SIGMA IRI) after the OGTT were significantly higher in t he obese women than in the controls. LPL, HL and the HL-to-LPL ratio w ere significantly higher in the obese women than in the controls. HL a nd LPL correlated positively with SIGMA glucose, SIGMA IRI and body ma ss index (BMI) and negatively with plasma triglycerides. Partial corre lation analysis demonstrated that, when exposed to similar SIGMA IRI v alues, HL and LPL were no longer correlated with SIGMA glucose, plasma triglycerides and BMI. HDL2 cholesterol levels were negatively correl ated with HL, posthepatic plasma lipolytic activity, SIGMA glucose, pl asma triglycerides and BMI. HDL2 cholesterol concentrations were direc tly correlated with LPL. Partial correlation analysis showed that when exposed to similar HL and LPL values, HDL2 cholesterol values were no longer correlated with SIGMA glucose, SIGMA IRI, plasma triglycerides and BMI. Therefore, our results demonstrate that the low HDL2 cholest erol levels found in obese women may be related to the high levels of HL and to the high HL-to-LPL ratio which in tum could be determined by the peripheral insulin resistance.