Pd. Hoagland et al., SIZE-EXCLUSION CHROMATOGRAPHY WITH VISCOSITY DETECTION OF COMPLEX POLYSACCHARIDES - COMPONENT ANALYSIS, Journal of agricultural and food chemistry, 41(8), 1993, pp. 1274-1281
Complex polysaccharides obtained from plants and microbes are finding
increased application in the food industry as additives to improve the
functional properties of processed foods. High-performance size exclu
sion chromatography (HPSEC) with concentration-viscosity detection, co
upled with Gaussian curve fitting of concentration and viscosity chrom
atograms, a method earlier developed to investigate the behavior of a
variety of pectins in solution, has now been applied to tragacanthin,
gum locust bean, (carboxymethyl)cellulose, sodium alginates, apple pec
tin, and gum arabic. Weight-average intrinsic viscosities (i.v.) were
determined directly from areas under the concentration and specific vi
scosity curves. In addition, global and component radii of gyration (R
(gw)) and molecular weights (MW(w)) were determined from both size and
universal calibration of columns with pululans. Gaussian component i.
v. and component R(gw) values of some polysaccharides, investigated in
0.05 M NaNO3 at 35-degrees-C, were found to be related by a character
istic power law exponent. HPSEC with concentration and viscosity detec
tion has gold potential for rapidly determining physical properties cr
ucial to control of quality of polysaccharides in the food industry.