IN-VITRO CHEMOSENSITIVITY TEST FOR HUMAN GENITOURINARY TUMORS USING COLLAGEN GEL MATRIX

An in vitro chemosensitivity test using collagen gel matrix assay with quantitative evaluation of cell viability using 3-(4,5-dimethylthiazo l-2-yl)-2,5-diphenyl tetrazolium bromide was applied to 40 human genit ourinary tumor specimens. Chemosensitivity was determined in 34 (85%) of the 40 specimens. The anticancer drugs tested against 14 renal cell carcinomas showed lower activities than against 15 transitional cell carcinomas with different sensitivity patterns among the individual sp ecimens demonstrated. Our assay system could be successfully applied t o human genitourinary tumors and can be used as a practical and highly reproducible chemosensitivity test in vitro.